Application
Western blot analysis of ARD1 expression in non-transfected 293T: (A), mouse ARD1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of ARD1 expression in MCF7 (A), HeLa (B) and HL-60 (C) whole cell lysates.| Product Name | ARD1 Antibody (FL-235) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-235 representing full length ARD1 of human origin |
| Modification Notes | The ARD1 subfamily of proteins belongs to the larger acetyltransferase family. N-terminal acetyltransferase complex ARD1, also designated Te2, forms a complex with NARG1, displaying N-terminal acetyltransferase activity. With-out NARG1, ARD1 promotes hypoxia-inducible factor-1a (HIF-1å) degradation by displaying internal acetyltransferase activity towards HIF-1å. This ubiquitously expressed protein, which is mainly cytoplasmic, is cleaved by caspases during apoptosis. ARD1 interacts with the ribosome, NARG1 and HIF-1å. In its binding to HIF-1å, ARD1 acts as a protein acetyltransferase by regulating its stability. In many cell lines, ARD1 is downregulated in response to hypoxia. ARD1 is expressed throughout the developing brain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of N-terminal Acetyltransferase Complex ARD1 Subunit Homolog of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and feline: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-235 representing full length ARD1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-235 representing full length ARD1 of human origin |
| Entrez Gene | 8260 |
|---|
Application
Western blot analysis of ARD1 expression in non-transfected 293T: (A), mouse ARD1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of ARD1 expression in MCF7 (A), HeLa (B) and HL-60 (C) whole cell lysates.| Application Notes | recommended for detection of N-terminal Acetyltransferase Complex ARD1 Subunit Homolog of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and feline: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
