Application
Western blot analysis of GAK expression in non-transfected: (A) and truncated human GAK transfected: (B) 293T whole cell lysates.| Product Name | GAK Antibody (R-360) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-360 representing the kinase domain of GAK (cyclin G associated kinase) of rat origin |
| Modification Notes | Cyclins are the regulatory subunits of Cdc2 p34 and related cyclin-dependent kinases (Cdks) which play critical roles in the control of cell cycle progression. The catalytic subunit for cyclin A and B is Cdc2 p34 kinase. The Cdc2-cyclin B complex controls the G2 to M transition whereas Cdc2-cyclin A regulates S phase progression (1,2). The G1 to S transition, however, appears to be controlled by the G1 cyclins (1,2). Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5 (3-5). Cyclin E and Cdk2 interact during the G1 to S transition (6). Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor (7). Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency (7). A serine/threonine kinase, designated GAK for cyclin G associated kinase, has been identified. GAK has been shown to bind directly to cyclin G and to co-immunoprecipitate with Cdk5, which also associates with cyclin G (8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for GAK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-360 representing the kinase domain of GAK (cyclin G associated kinase) of rat origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-360 representing the kinase domain of GAK (cyclin G associated kinase) of rat origin |
| Entrez Gene | 2580 |
|---|
Application
Western blot analysis of GAK expression in non-transfected: (A) and truncated human GAK transfected: (B) 293T whole cell lysates.| Application Notes | recommended for GAK of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
