Application
Antibodies tested include GAL4 (DBD) (RK5C1): tested at 1.0 µg/ml (A), 0.1 µg/ml (B) and 0.01 µg/ml (C) and GAL4 (DBD): tested at 0.1 µg/ml (D) and 0.01 µg/ml (E).Application
Western blot analysis of yeast recombinant GAL4 DBD fusion protein.| Product Name | GAL4 Antibody (DBD) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-147 mapping within the N-terminal DNA binding domain of GAL4 |
| Modification Notes | The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators (1,2). Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation (3), this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells (4,5). A unique “two-hybrid” system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISAGSChIP |
| recommended for detection of GAL4 DNA binding domain by WB, IP and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-147 mapping within the N-terminal DNA binding domain of GAL4 |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-147 mapping within the N-terminal DNA binding domain of GAL4 |
Application
Antibodies tested include GAL4 (DBD) (RK5C1): tested at 1.0 µg/ml (A), 0.1 µg/ml (B) and 0.01 µg/ml (C) and GAL4 (DBD): tested at 0.1 µg/ml (D) and 0.01 µg/ml (E).
Application
Western blot analysis of yeast recombinant GAL4 DBD fusion protein.| Application Notes | recommended for detection of GAL4 DNA binding domain by WB, IP and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
