Application
Western blot analysis of Neurabin-I expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Product Name | Neurabin-I Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 799-1098 mapping at the C-terminus of Neurabin-I of human origin |
| Modification Notes | Brain-specific neurabin I (neural tissue-specific F-actin binding protein I) is highly concentrated in the synapse of developed neurons; it localizes in the growth cone lamellipodia during neuronal development (1). Suppression of endogenous neurabin in rat hippocampal neurons inhibits neurite formation (1). Neurabin I recruits active PP1 via a PP1-docking sequence; mutation of the PP1-binding motif halts filopodia and restores stress fibers in neurabin I-expressing cells (2,3). Neurabin II (Spinophilin) is ubiquitously expressed but is abundantly expressed in brain (4). Neurabin II localizes to neuronal dentritic spines, which are the specialized protrusions from dendritic shafts that receive most of the excitatory input in the CNS (5). Neurabin II may regulate dendritic spine properties as neurabin II(-) mice have increased spine density during development in vitro and exhibit altered filopodial formation in cultured cells (5). Neurabin may also play a role in glutamatergic transmission as Neurabin II(-) mice exhibit reduced long-term depression and resistance to kainate-induced seizures and neronal apoptosis (5). Neurabin II complexes with the catalytic subunit of protein phosphatase-1 (PP1) in vitro thus modulating the activity of PP1 (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Neurabin-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 799-1098 mapping at the C-terminus of Neurabin-I of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 799-1098 mapping at the C-terminus of Neurabin-I of human origin |
| Entrez Gene | 55607 |
|---|
Application
Western blot analysis of Neurabin-I expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human hippocampus tissue showing cytoplasmic staining of neuronal cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.| Application Notes | recommended for detection of Neurabin-I of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
