Application
Western blot analysis of NRAMP 2 expression in mouse brain tissue extract.| Product Name | NRAMP 2 Antibody (H-108) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 461-568 mapping at the C-terminus of NRAMP 2 of human origin |
| Modification Notes | Natural resistance associated macrophage proteins (NRAMPs) belong to a super-family of highly conserved integral membrane proteins. NRAMP 1 is an intracellular macrophage protein located at the phagosomal membrane where it functions as a divalent cation transporter for Fe2+, Zn2+ and Mn2+ (1,2). NRAMP 1 is a pH-dependent antiporter that transports metal ions either into or out of the phagosome against a proton gradient (1). The gene encoding human NRAMP 1 maps to chromosome 2q35 (3). In humans, polymorphisms in the NRAMP 1 gene are linked to susceptibility to M. tuberculosis and leprosy (4-6). NRAMP 2 is another divalent cation transporter ubiquitously expressed as two splice variants, which are distinguished by the presence (isoform 1) or absence (isoform 2) of an iron response element (7,8). In the duodenum of the small intestine, dietary iron regulates NRAMP 2 expression at the brush border (9). The gene encoding human NRAMP 2 maps to chromosome 12q13 (8). Mutations in the gene for NRAMP 2 in mice and rats result in severe anemia (10). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NRAMP 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 461-568 mapping at the C-terminus of NRAMP 2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 461-568 mapping at the C-terminus of NRAMP 2 of human origin |
| Entrez Gene | 4891 |
|---|
Application
Western blot analysis of NRAMP 2 expression in mouse brain tissue extract.| Application Notes | recommended for detection of NRAMP 2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
