Application
Western blot analysis of CD34 expression in TF-1 whole cell lysate.Application
Western blot analysis of CD34 expression in non-transfected 293T: (A), human CD34 transfected 293T: (B) and TF-1 (C) whole cell lysates.Application
Western blot analysis of CD34 expression in non-transfected control (A) and CD34 siRNA transfected (B) HeLa cells. Blot probed with CD34 (H-140): . Lamin A/C (H-110): used as specificity and loading control.Application
Western blot analysis of CD34 expression in Hs68 whole cell lysate.| Product Name | CD34 Antibody (H-140) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 151-290 of CD34 of human origin |
| Modification Notes | CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD34 of mouse, human and, to a lesser extent rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 151-290 of CD34 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 151-290 of CD34 of human origin |
| Entrez Gene | 947 |
|---|
Application
Western blot analysis of CD34 expression in TF-1 whole cell lysate.
Application
Western blot analysis of CD34 expression in non-transfected 293T: (A), human CD34 transfected 293T: (B) and TF-1 (C) whole cell lysates.
Application
Western blot analysis of CD34 expression in non-transfected control (A) and CD34 siRNA transfected (B) HeLa cells. Blot probed with CD34 (H-140): . Lamin A/C (H-110): used as specificity and loading control.
Application
Western blot analysis of CD34 expression in Hs68 whole cell lysate.| Application Notes | recommended for detection of CD34 of mouse, human and, to a lesser extent rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
