Application
Western blot analysis of ARK-2 expression in non-transfected 293T: (A), mouse ARK-2 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Western blot analysis of human recombinant ARK-2.Application
Western blot analysis of ARK-2 expression in NIH/3T3 whole cell lysate (A) and mouse thymus (B) and rat thymus (C) tissue extracts.| Product Name | ARK-2 Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-75 mapping at the N-terminus of ARK-2 of human origin |
| Modification Notes | Aurora related kinase-1 (ARK-1, STK15, Aurora2, Aik1) and -2 (ARK-2, STK12, Aurora1) are centrosome-associated serine/ threonine kinases that regulate centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis (1-3). ARK-1 and -2 are expressed in the nucleus and localize to distinct portions of mitotic machinery such as the centrosome, spindle poles (ARK-1), and midbody (ARK-2) during mitosis (3,4). ARK-1 and -2 transcripts are present at high levels in human thymus and fetal liver (1). ARK-1 protein has elevated expression in colon carcinoma lines (HT-29, SNU-C2B, COLO 205, SW480, 837 and 948) and accumulates during metaphase in HeLa cells (1,5). ARK-2 protein levels are maximal during both S and G2/M phases, whereas ARK-1 protein is degraded after G2/M via the ubiquitin-proteasome pathway (6,7). ARK-2 has a unique genetic loci relative to ARK-1, suggesting that these two kinases, with oncogenic potential, have different roles in cell cycle progression (1,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ARK-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-75 mapping at the N-terminus of ARK-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-75 mapping at the N-terminus of ARK-2 of human origin |
| Entrez Gene | 9212 |
|---|
Application
Western blot analysis of ARK-2 expression in non-transfected 293T: (A), mouse ARK-2 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Western blot analysis of human recombinant ARK-2.
Application
Western blot analysis of ARK-2 expression in NIH/3T3 whole cell lysate (A) and mouse thymus (B) and rat thymus (C) tissue extracts.| Application Notes | recommended for detection of ARK-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
