Application
Antibodies tested include p-cPLA2 (Ser 505): (A,B,C) and cPLA2 (C-20): (D,E,F).Application
Western blot analysis of cPLA2 phosphorylation in non-transfected: (A), untreated mouse cPLA2 transfected: (B) and lambda protein phosphatase treated mouse cPLA2 transfected: (C) 293T whole cell lysates.Application
Western blot analysis of cPLA2 phosphorylation in NIH/3T3 whole cell lysate.Application
Western blot analysis of cPLA2 phosphorylation in non-transfected: (A) and mouse cPLA2 transfected: (B) 293T whole cell lysates.| Product Name | p-cPLA2 Antibody (Ser 505) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated near the C-terminus of cPLA2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycero-phospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate. Residue Ser 505 of cPLA2 is a consensus site for phosphorylation by MAPK, but is also phosphorylated by PI-3 kinase via a pathway not involving MAPK. Dual phosphorylation of Ser 505 and Ser 727 by ERK1/2 and/or p38 MAP kinase (mainly the 2a isoform) is required for activation of cPLA2 and acts synergistically with the increase in intracellular Ca2+. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of cytosolic PLA2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated near the C-terminus of cPLA2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated near the C-terminus of cPLA2 of human origin |
| Entrez Gene | 5321 |
|---|
Application
Antibodies tested include p-cPLA2 (Ser 505): (A,B,C) and cPLA2 (C-20): (D,E,F).
Application
Western blot analysis of cPLA2 phosphorylation in non-transfected: (A), untreated mouse cPLA2 transfected: (B) and lambda protein phosphatase treated mouse cPLA2 transfected: (C) 293T whole cell lysates.
Application
Western blot analysis of cPLA2 phosphorylation in NIH/3T3 whole cell lysate.
Application
Western blot analysis of cPLA2 phosphorylation in non-transfected: (A) and mouse cPLA2 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of cytosolic PLA2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
