Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Aldose Reductase expression in non-transfected: (A) and human Aldose Reductase transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Aldose Reductase expression in non-transfected 293T: (A), mouse Aldose Reductase transfected 293T: (B) and JAR (C) whole cell lysates.Application
Western blot analysis of Aldose Reductase expression in non-transfected 293: (A), human Aldose Reductase transfected 293: (B) and HeLa (C)whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic and nuclear staining of cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Aldose Reductase expression in and Caki-1 whole cell lysate.| Product Name | Aldose Reductase Antibody (FL-316) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-316 representing full length Aldose Reductase of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Aldose reductase (designated ALR2) is member of the monomeric NADPH-dependent aldoketoreductase family. ALR2 catalyzes the reduction of various aldehydes and has been implicated in the development of diabetic complications by catalyzing the reduction of the aldehyde form of glucose, to the corresponding sugar alcohol, sorbitol. This pathway plays a minor role in glucose metabolism in most tissues, however in diabetic hyperglycemia, cells undergoing insulin-independent uptake of glucose accumulate significant quantities of sorbitol. The resulting hyperosmotic stress to cells may be a cause of diabetic complications such as neuropathy, retinopathy, and cataracts. Aldose reductase is very similar to human aldehyde reductase (designated ALR1), bovine prostaglandin F synthase and to the European common frog protein, rho-crystallin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-316 representing full length Aldose Reductase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-316 representing full length Aldose Reductase of human origin |
| Entrez Gene | 231 |
|---|
Application
Immunofluores-cence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Aldose Reductase expression in non-transfected: (A) and human Aldose Reductase transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Aldose Reductase expression in non-transfected 293T: (A), mouse Aldose Reductase transfected 293T: (B) and JAR (C) whole cell lysates.
Application
Western blot analysis of Aldose Reductase expression in non-transfected 293: (A), human Aldose Reductase transfected 293: (B) and HeLa (C)whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic and nuclear staining of cortical cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Aldose Reductase expression in and Caki-1 whole cell lysate.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:5~1:20 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
