Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear and cytoplasmic staining of Islets of Langerhans and glandular cells.Application
Western blot analysis of p14 ARF expression in BJAB whole cell lysate.| Product Name | p14 ARF Antibody (H-132) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-132 of p14 ARF of human origin |
| Modification Notes | The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates promotes orderly progression through the cell cycle. Multiple proteins are encoded by the tumor suppressor gene CDKN2A (MTS1/ p16INK4a) via translation of alternate reading frames, resulting in the production of the p19 ARF protein in mice and the p14 ARF protein in humans. p14 ARF induces an increase in MDM2 and p21 levels and leads to cell cycle arrest in both G1 and G2/M. p14 ARF is negatively regulated by p53 and is known to bind directly to MDM2. CDKN2A also encodes the mitotic protein p16, which binds to and inhibits the Cdk4/cyclin D complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of p14 ARF of human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-132 of p14 ARF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-132 of p14 ARF of human origin |
| Entrez Gene | 1029 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing nuclear and cytoplasmic staining of Islets of Langerhans and glandular cells.
Application
Western blot analysis of p14 ARF expression in BJAB whole cell lysate.| Application Notes | recommended for detection of p14 ARF of human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
