Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of BMP-1 expression in NIH/3T3 whole cell lysate.| Product Name | BMP-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 687-986 mapping at the C-terminus of BMP-1 of human origin |
| Modification Notes | Bone morphogenic proteins (BMPs) are members of the TGF∫ superfamily (1,2). BMPs are involved in the induction of cartilage and bone formation (3). In vivo studies have shown that BMP-2 (also designated BMP-2A) and BMP-3 can independently induce cartilage formation (3). BMP-1 is a metalloprotease that plays important roles in regulating the deposition of fibrous extracellular matrix in vertebrates, including provision of the procollagen C-proteinase activity that processes the major fibrillar collagens I-III (3). Smad3 association with the TGF∫ receptor complex and Smad1 translocation to the nucleus are observed after the addition of BMP-4 (also designated BMP-2B), suggesting that BMP-4 may play a role in activation of the Smad pathway (4,5). BMP-5, BMP-6 and BMP-7 all share high sequence homology with BMP-2, indicating that they each may be able to induce cartilage formation (6,7). BMP-8 (also designated OP-2) is thought to be involved in early development, as detectable expression has not been found in adult organs (8). Human BMP-1 splice variants occur between residues 703 and 986 and include: BMP1-1, BMP1-4, BMP1-5, BMP1-6, BMP1-7 (4,10). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of BMP-1 and Tolloid-like protein 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 687-986 mapping at the C-terminus of BMP-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 687-986 mapping at the C-terminus of BMP-1 of human origin |
| Entrez Gene | 649 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of BMP-1 expression in NIH/3T3 whole cell lysate.| Application Notes | recommended for detection of BMP-1 and Tolloid-like protein 1 and 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
