Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of ValRS expression in HeLa whole cell lysate.| Product Name | ValRS Antibody (H-203) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-203 mapping at the N-terminus of ValRS of human origin |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. ValRS (valyl-tRNA synthetase), also known as Protein G7a, belongs to the class-I aminoacyl-tRNA synthetase family that includes the related proteins, LeuRS and IleRS. These proteins are large monomeric proteins and play a major role in catalyzing the aminoacylation of tRNA by their cognate amino acid. ValRS joins Val to tRNA(Val) at its synthetic active site. At its CP1 editing active site, ValRS hydrolyzes or deacylates tRNA(Thr) that is incorrectly joined to Val. ValRS forms aggregates with EF-1 (elongation factor 1) and, via this complex, catalyzes the aminoacylation of tRNA and its transfer to EF-1. In addition, ValRS may be regulated by PKC-dependent phosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ValRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-203 mapping at the N-terminus of ValRS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-203 mapping at the N-terminus of ValRS of human origin |
| Entrez Gene | 7407 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of ValRS expression in HeLa whole cell lysate.| Application Notes | recommended for detection of ValRS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
