Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Dab1 expression in non-transfected: (A) and human Dab1 transfected: (B) 293T whole cell lysates.| Product Name | Dab1 Antibody (H-103) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 451-553 mapping at the C-terminus of Disabled 1 (Dab1) of human origin |
| Modification Notes | Dab1, a homolog of the Drosophila Disabled protein, is an adaptor protein involved in neural development. This cytoplasmic protein is tyrosine-phosphorylated during rapid expansion of the developing nervous system, and it is thought to interact with other proteins via a domain similar to the PTB domains of the Shc family. Dab1 has been shown to interact with the SH2 domains of Src, Fyn and Abl (1). Mutations in Dab1 result in widespread abnormalities in the brain, similar to those seen in Reelin mutants (2). Reelin is a secreted protein thought to play a role in directing migrating neurons (3). Evidence suggests that Dab1 functions downstream of Reelin in a signaling pathway involved in positioning cells in the developing brain (4). Dab2 (also designated DOC-2) is a mitogen-responsive phosphoprotein that binds the SH3 domain of Grb2, and it is thought to be a negative regulator of growth (5). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Dab1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 451-553 mapping at the C-terminus of Disabled 1 (Dab1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 451-553 mapping at the C-terminus of Disabled 1 (Dab1) of human origin |
| Entrez Gene | 1600 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at high magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Dab1 expression in non-transfected: (A) and human Dab1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Dab1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
