Application
Western blot analysis of Exo1 expression in non-transfected: (A) and human Exo1 transfected: (B) 293T whole cell lysates and mouse testis tissue extract (C).Application
Western blot analysis of Exo1 expression in non-transfected: (A) and human Exo1 transfected: (B) 293T whole cell lysates and mouse testis tissue extract (C).| Product Name | Exo1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 11-310 mapping near the N-terminus of Exo1 of human origin |
| Modification Notes | Comparative evaluation of the expression patterns of the human and mouse genes, combined with previous biochemical and yeast genetic studies, indicate that the Exo1 (Exonuclease I) proteins are important contributors to chromosome processing during mammalian DNA repair and recombination. In mice, the Exo1 gene maps to distal chromosome 1, consistent with the recent mapping of the orthologous human HEX1/EXO1 gene to chromosome 1q43. Exo1 is expressed prominently in testis, an area of active homologous recombination, and spleen, a prominent lymphoid tissue. In both mammalian and yeast systems, Exo1 is a 5'-3' double stranded DNA exonuclease that has previously been implicated in DNA mismatch repair (MMR). The MMR system ensures genome integrity by removing mispaired and unpaired bases that originate during replication. In humans, Exo1 interacts with MSH2 and MLH1 and has been proposed to be a redundant exonuclease in MMR. In both mammalian and yeast systems, Exo1 plays a structural role in MMR and stabilizes multiprotein complexes containing a number of MMR proteins. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Exo1a and Exo1b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 11-310 mapping near the N-terminus of Exo1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 11-310 mapping near the N-terminus of Exo1 of human origin |
| Entrez Gene | 9156 |
|---|
Application
Western blot analysis of Exo1 expression in non-transfected: (A) and human Exo1 transfected: (B) 293T whole cell lysates and mouse testis tissue extract (C).
Application
Western blot analysis of Exo1 expression in non-transfected: (A) and human Exo1 transfected: (B) 293T whole cell lysates and mouse testis tissue extract (C).| Application Notes | recommended for detection of Exo1a and Exo1b of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
