Application
Western blot analysis of Dynactin p62 expression in RAW 264.7 whole cell lysate.Application
Western blot analysis of Dynactin p62 expression in BC3H1 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Dynactin p62 expression in Sol8 whole cell lysate.| Product Name | Dynactin p62 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 161-460 mapping at the C-terminus of Dynactin p62 of human origin |
| Modification Notes | Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein (1). Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end (2-5). The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1 (2). Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (1). In addition, Dynactin p62 is distributed in the nucleus at very high expression levels (1). Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton (2-4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dynactin p62 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 161-460 mapping at the C-terminus of Dynactin p62 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 161-460 mapping at the C-terminus of Dynactin p62 of human origin |
| Entrez Gene | 51164 |
|---|
Application
Western blot analysis of Dynactin p62 expression in RAW 264.7 whole cell lysate.
Application
Western blot analysis of Dynactin p62 expression in BC3H1 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Dynactin p62 expression in Sol8 whole cell lysate.| Application Notes | recommended for detection of Dynactin p62 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
