Application
Western blot analysis of CCS expression in non-transfected 293T: (A), mouse CCS transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of CCS expression in HL-60 (A), Hep G2 (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of CCS expression in non-transfected control (A) and CCS siRNA transfected (B) HeLa cells. Blot probed with CCS (FL-274): . Actin (I-19): used as specificity and loading control.Application
Western blot analysis of CCS expression in HeLa whole cell lysate.| Product Name | CCS Antibody (FL-274) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-274 representing full length CCS of human origin |
| Modification Notes | Cu-Zn superoxide dismutase-1 (SOD-1) is a well characterized cytosolic scavenger of oxygen free radicals that requires copper and zinc binding to potentiate its enzymatic activity. Copper chaperone for SOD-1 (CCS) is essential for the incorporation of copper into SOD-1, and therefore is necessary for its enzymatic activity. CCS prevents copper ions from binding to intracellular copper scavengers and provides the SOD-1 enzyme with the necessary copper cofactor. CCS escorts copper only to SOD-1 and fails to deliver copper to proteins in the mitochondria, nucleus or secretory pathway. CCS interacts with both wildtype and mutated forms of SOD-1 through CCS domains that are homologous in SOD-1. CCS exists as a homodimer that may form a heterodimer with SOD-1 during copper loading. While many tissues express CCS, the chaperone is most abundant in the kidney, liver and Purkinje cells in the neurophil of the central nervous system. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CCS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-274 representing full length CCS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-274 representing full length CCS of human origin |
| Entrez Gene | 9973 |
|---|
Application
Western blot analysis of CCS expression in non-transfected 293T: (A), mouse CCS transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of CCS expression in HL-60 (A), Hep G2 (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of CCS expression in non-transfected control (A) and CCS siRNA transfected (B) HeLa cells. Blot probed with CCS (FL-274): . Actin (I-19): used as specificity and loading control.
Application
Western blot analysis of CCS expression in HeLa whole cell lysate.| Application Notes | recommended for detection of CCS of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
