Application
Western blot analysis of SWAP-70 expression in non-transfected (A) and human SWAP-70 transfected (B) 293 whole cell lysates.Application
Western blot analysis of SWAP-70 expression in Hep G2 whole cell lysate.| Product Name | SWAP-70 Antibody (Z-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SWAP-70 of human origin by WB, IP and ELISA |
| Modification Notes | SWAP-70 is a protein that is part of a protein complex that catalyzes cell-free DNA recombination between immunoglobulin (Ig) heavy chain gene switch region substrates. In resting B lymphocytes, SWAP-70 is localized mainly in the cytoplasm, however, in activated B cells, SWAP-70 is recruited to the plasma membrane and then translocates to the nucleus. In the nucleus, SWAP-70 recognizes specific switch regions, acting as a switch recombinase and causing a DNA break. The cellular and intracellular localization before and after B-cell activation also suggests a role for SWAP-70 in signaling in B cell activation. In addition, SWAP-70 contains three nuclear localization signals, has a weak affinity for DNA, binds ATP, and forms specific, high affinity complexes with B23, C23, and poly (ADP-ribose) polymerase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SWAP-70 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SWAP-70 of human origin by WB, IP and ELISA |
| Entrez Gene | 23075 |
|---|
Application
Western blot analysis of SWAP-70 expression in non-transfected (A) and human SWAP-70 transfected (B) 293 whole cell lysates.
Application
Western blot analysis of SWAP-70 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
