Application
Western blot analysis of HS2ST1 expression in non-transfected (A) and human HS2ST1 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.Application
Western blot analysis of HS2ST1 expression in SK-N-MC whole cell lysate.| Product Name | HS2ST1 Antibody (Z-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of HS2ST1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Heparan sulfate structures, which are responsible for executing multiple biologic activities, are generated and regulated by heparan sulfate biosynthetic enzymes. HS2ST1 (heparan sulfate 2-O-sulfotransferase 1), also known as HS2ST, is a 356 amino acid single-pass type II membrane protein that localizes to the golgi apparatus and belongs to the sulfotransferase 3 family. Expressed as multiple alternatively spliced isoforms, HS2ST1 functions to catalyze the transfer of sulfate groups to hexuronic acid residues within maturing heparan sulfate (HS), an event which is crucial for proper HS-related ligand binding and signaling processes. HS2ST1 is subject to post-translational N-glycosylation and, in addition to its role in HS function, may be involved in proper kidney formation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of HS2ST1 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of HS2ST1 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 9653 |
|---|
Application
Western blot analysis of HS2ST1 expression in non-transfected (A) and human HS2ST1 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human brain tissue showing cytoplasmic localization.
Application
Western blot analysis of HS2ST1 expression in SK-N-MC whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
