| Product Name | HM74 Antibody (H-64) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 300-363 mapping within a C-terminal cytoplasmic domain of HM74A of human origin |
| Modification Notes | HM74, also known as PUMAG or Puma-g, is a member of the G protein coupled receptor (GPCR) superfamily. In humans, HM74 is encoded by two different genes (GPR109A and GPR109B) that produce proteins, namely HM74A and HM74 (or HM74B), which are 96% homologous. In mice and rats, only one gene encodes the HM74 protein (Gpr109a). HM74 is a G(i) protein-coupled receptor that mediates the metabolic effects of nicotinic acid. Localizing to the cell membrane, HM74 is highly expressed in adipocytes, immune cells and spleen. Like all members of the GPCR superfamily, HM74 contains seven transmembrane domains. HM74 lacks the N-linked glycosylation sites near the N-terminus that are present in other GPCR family members. Furthermore, HM74 shows a more diverged amino acid sequence homology from most family members, implying different ligand specificity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of HM74A and HM74B of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 300-363 mapping within a C-terminal cytoplasmic domain of HM74A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 300-363 mapping within a C-terminal cytoplasmic domain of HM74A of human origin |
| Application Notes | recommended for detection of HM74A and HM74B of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
