Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic and membrane localization.Application
Western blot analysis of Dkk-2 expression in non-transfected (A) and human Dkk-2 transfected (B) 293 whole cell lysates.Application
Western blot analysis of Dkk-2 expression in Jurkat whole cell lysate.| Product Name | Dkk-2 Antibody (Q-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Dkk-2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | The Wnt genes are a group of well conserved, cysteine-rich secreted glycoproteins that are required for numerous develop-mental processes including embryogenesis, asymmetric cell division and central nervous system (CNS) patternins. Wnt association with the seven membrane spanning receptor Frizzled, activates Dishevelled, which downregulates glycogen synthase kinase (GSK) through serine phosphorylation, causing the accumulation of b-Catenin and subsequent regulation of developmentally significant Wnt target genes. The Dickkopf family of secreted inhibitors of Wnt signaling ensures proper morphological development by antagonizing different stages of the Wnt cascade. Dkk-2 (Dickkopf-2) is a 259-amino acid secreted protein that is composed of an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a 50-55-amino acid linker region. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Dkk-2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Dkk-2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 27123 |
|---|
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic and membrane localization.
Application
Western blot analysis of Dkk-2 expression in non-transfected (A) and human Dkk-2 transfected (B) 293 whole cell lysates.
Application
Western blot analysis of Dkk-2 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
