Application
Western blot analysis of GABAB R2 expression in non-transfected: (A) and human GABAB R2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GABAB R2 expression in mouse brain tissue extract.| Product Name | GABAB R2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 183-482 mapping within an extracellular domain of GABAB R2 of human origin |
| Modification Notes | In the central nervous system (CNS), gamma-aminobutyric acid (GABA) is the main inhibitory neurotransmitter that functions to regulate neuronal firing. GABA exerts its effects through two different kinds of receptors: ionotropic receptors (GABAA R and GABAC R), which produce fast inhibitory signals, and metabotropic receptors (GABAB R), which produce slow inhibitory signals. The GABAB R receptor is a heterodimer that consists of two multi-pass membrane proteins, designated GABAB R1 and GABAB R2, both of which belong to the G protein-coupled receptor family and are highly expressed in brain tissue. Together, GABAB R1 and GABAB R2 play a crucial role in the fine-tuning of inhibitory synaptic transmissions and are implicated in slow wave sleep, muscle relaxation, hippocampal long-term potentiation and antinociception events. Both GABAB R1 and GABAB R2 are regulated by G proteins that have a variety of functions, including activation of potassium channels, inhibition of adenylyl cyclase (A cyclase) activity and modulation of inositol phospholipid hydrolysis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GABAB R2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 183-482 mapping within an extracellular domain of GABAB R2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 183-482 mapping within an extracellular domain of GABAB R2 of human origin |
| Entrez Gene | 9568 |
|---|
Application
Western blot analysis of GABAB R2 expression in non-transfected: (A) and human GABAB R2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GABAB R2 expression in mouse brain tissue extract.| Application Notes | recommended for detection of GABAB R2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
