Application
Western blot analysis of Rhodanese expression in non-transfected: (A) and human Rhodanese transfected: (B) 293 whole cell lysates and mouse liver tissue extract (C).Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Rhodanese siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Rhodanese (H-75): .Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.Application
Western blot analysis of Rhodanese expression in non-transfected control (A) and Rhodanese siRNA transfected (B) HeLa cells. Blot probed with Rhodanese (H-75): . Actin (I-19): used as specificity and loading control.Application
Western blot analysis of Rhodanese expression in mouse (A) and rat (B) liver tissue extracts.| Product Name | Rhodanese Antibody (H-75) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 36-110 mapping near the N-terminus of Rhodanese of human origin |
| Modification Notes | Rhodanese (also known as thiosulfate sulfurtransferase) is a mitochondrial matrix enzyme that is encoded by the nucleus (1). Rhodanese is a 297-residue polypeptide and has been proposed to play roles in cyanide detoxification, the formation of iron-sulfur proteins, and the modification of sulfur-containing enzymes (1,2). Rhodanese was first identified in human red cells in 1956 and has been crystallized from beef liver (1). In mammals, most cyanide is converted to thiocyanate by the rhodanese (3). There is an association between Leber’s optic neuropathy and deficiency of rhodanese activity in liver and rectal mucosa (4). Greatly reduced activity of this enzyme has been observed in the livers of two males with Leber optic atrophy from a well-studied Swiss family with five symptomatic persons in four generations (3). The red cell and tissue rhodanese are determined by separate genes, but more than one locus may be concerned with the synthesis of heterogeneous tissue isozymes (5). The gene which encodes rhodanese maps to human chromosome 22q11.2-qter (2). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Rhodanese of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 36-110 mapping near the N-terminus of Rhodanese of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 36-110 mapping near the N-terminus of Rhodanese of human origin |
| Entrez Gene | 7263 |
|---|
Application
Western blot analysis of Rhodanese expression in non-transfected: (A) and human Rhodanese transfected: (B) 293 whole cell lysates and mouse liver tissue extract (C).
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Rhodanese siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Rhodanese (H-75): .
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing cytoplasmic localization.
Application
Western blot analysis of Rhodanese expression in non-transfected control (A) and Rhodanese siRNA transfected (B) HeLa cells. Blot probed with Rhodanese (H-75): . Actin (I-19): used as specificity and loading control.
Application
Western blot analysis of Rhodanese expression in mouse (A) and rat (B) liver tissue extracts.| Application Notes | recommended for detection of Rhodanese of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
