Application
Western blot analysis of ephrin-B1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat testis tissue extracts.| Product Name | p-ephrin-B1 Antibody (Tyr 317) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 300 phosphorylated ephrin-B1 of Xenopus laevis origin, correspondingly Tyr 317 phosphorylated ephrin-B1 of human origin and correspondingly Tyr 316 phosphorylated ephrin-B1 of mouse and rat origin of mouse, rat, human, Xeno |
| Modification | Phosphoralated |
| Modification Notes | Ephrins, which act as ligands for Eph receptors, are cell-surface proteins which fall into two categories, ephrin-A and ephrin-B, based on their structure and function. Ephrin-B proteins are transmembrane and have conserved cytoplasmic tyrosine residues that are phosphorylated upon interaction with an EphB receptor. Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development. Mouse, rat, human and Xenopus laevis ephrin-B1 are phosphorylated on Tyr 317 in vivo, which may be induced by a receptor tyrosine kinase. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIP |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 300 phosphorylated ephrin-B1 of Xenopus laevis origin correspondingly Tyr 317 phosphorylated ephrin-B1 of human origin and correspondingly Tyr 316 phosphorylated ephrin-B1 of mouse and rat origin of mouse rat human Xeno |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 300 phosphorylated ephrin-B1 of Xenopus laevis origin, correspondingly Tyr 317 phosphorylated ephrin-B1 of human origin and correspondingly Tyr 316 phosphorylated ephrin-B1 of mouse and rat origin of mouse, rat, human, Xenopus laevis and zebrafish origin by WB and IP; also reactive with additional species, including bovine and canine |
| Entrez Gene | 1947 |
|---|
Application
Western blot analysis of ephrin-B1 phosphorylation in untreated (A) and lambda protein phosphatase-treated (B) rat testis tissue extracts.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
