Application
Western blot analysis of CLIC4 expression in non-transfected (A) and human CLIC4 transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | CLIC4 Antibody (U-100) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CLIC4 of human origin by WB , IP, IF, IHC(P) and ELISA |
| Modification Notes | CLIC4 (chloride intracellular channel 4), also known as H1, huH1, p64H1, CLIC4L or MTCLIC, is a 253 amino acid single-pass membrane protein that localizes to both the nucleus and the cytoplasm and contains one GST C-terminal domain. Expressed in placenta, heart and skeletal muscle, as well as in epithelial cells from kidney, colon and esophageal tissue, CLIC4 functions as a monomer that is able to form selective ion channels in target proteins, thereby facilitating the transport of chloride and other ions. The gene encoding CLIC4 maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson’s disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CLIC4 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of CLIC4 of human origin by WB , IP, IF, IHC(P) and ELISA |
| Entrez Gene | 25932 |
|---|
Application
Western blot analysis of CLIC4 expression in non-transfected (A) and human CLIC4 transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
