Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.Application
Western blot analysis of VDAC1/2/3 expression in HL-60 (A) and Jurkat (B) whole cell lysates.| Product Name | VDAC1/2/3 Antibody (FL-283) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-283 representing full length VDAC3 of human origin |
| Modification Notes | Adenine nucleotide translocator (ANT) and the voltage-dependent anion-selective channel proteins 1, 2 and 3 (VDAC1, VDAC2 and VDAC3) are components of the permeability transition pore complex (PTPC) of the mitochondrial inner or outer membranes. Formation of PTPCs, the subsequent dissipation of mitochondrial inner membrane potential and release of cytochrome c through the outer mitochondrial membrane are critical events in the early stages of apoptosis. Bax, a proapoptotic protein, has been shown to act upon ANT to induce the dissipation of mitochondrial inner membrane potential. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of VDAC1, VDAC2, VDAC3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-283 representing full length VDAC3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-283 representing full length VDAC3 of human origin |
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing cytoplasmic staining of myocytes.
Application
Western blot analysis of VDAC1/2/3 expression in HL-60 (A) and Jurkat (B) whole cell lysates.| Application Notes | recommended for detection of VDAC1, VDAC2, VDAC3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
