Application
Western blot analysis of Nopp140 expression in non-transfected: (A) and human Nopp140 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Nopp140 expression in non-transfected 293T: (A), mouse Nopp140 transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | Nopp140 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 620-699 mapping at the C-terminus of Nopp140 of human origin |
| Modification Notes | Nopp140, previously named p130, is a nucleolar phosphoprotein that has been shown to exist in multiple forms with different sizes. Nopp140 functions both as a chaperone for import and/or export from the nucleolus and as a transcription factor. Nopp140 was originally identified from rat liver as an NLS (nuclear localization signal)-binding protein, and has been further characterized as an RNAP (RNA Polymerase)-interacting protein. Nopp140 also associates with the general transcription factor TFIIB, and the protein kinase casein kinase II (CKII). CKII heavily phosphorylates Nopp140 to mediate binding of Nopp140 to NLS. Nopp 140 colocalizes with another nucleolar protein, NAP57, in the nucleolus and coiled bodies, and is thought to be involved in activities carried out within the nucleolus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Nopp140 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 620-699 mapping at the C-terminus of Nopp140 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 620-699 mapping at the C-terminus of Nopp140 of human origin |
| Entrez Gene | 9221 |
|---|
Application
Western blot analysis of Nopp140 expression in non-transfected: (A) and human Nopp140 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Nopp140 expression in non-transfected 293T: (A), mouse Nopp140 transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of Nopp140 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
