Application
Antibodies tested include p-Tau (Ser 396): (A,B) and Tau (C-17): (C,D).Application
Western blot analysis of Tau phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.Application
Blots were probed with p-Tau (Ser 396): preincubated with cognate phosphorylated peptide (A) and p-Tau (Ser 396): (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded rat hippocampal tissue showing cytoskeletal staining.| Product Name | p-Tau Antibody (Ser 396) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 396 of Tau of human origin |
| Modification | Phosphoralated |
| Modification Notes | Tau, also known as MAPT (microtubule-associated protein tau), MAPTL, MTBT1 or TAU, is a 758 amino acid protein that localizes to the cytoplasm, as well as to the cytoskeleton and the cell membrane, and contains four Tau/MAP repeats. Expressed in neuronal tissue and existing as multiple alternatively spliced isoforms, Tau functions to promote microtubule assembly and stability and is thought to be involved in the maintenance of neuronal polarity. Tau may also link microtubules with neural plasma membrane components and, in addition to its role in microtubule stability, is also necessary for cytoskeletal plasticity. Tau is highly subject to a variety of post-translational modifications, including phosphorylation on serine and threonine residues, polyubiquitination (and subsequent proteasomal degradation) and glycation of specific Tau isoforms. Defects in the gene encoding Tau are associated with Alzheimers disease, pallido-ponto-nigral degeneration (PPND), corticobasal degeneration (CBD) and progressive supranuclear palsy (PSP). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 396 phosphorylated Tau of human origin, correspondingly phosphorylated Ser 687 of mouse origin and correspondingly phosphorylated Ser 706 of rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 396 of Tau of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 396 of Tau of human origin |
| Entrez Gene | 4137 |
|---|
Application
Antibodies tested include p-Tau (Ser 396): (A,B) and Tau (C-17): (C,D).
Application
Western blot analysis of Tau phosphorylation in untreated (A) and lambda protein phosphatase treated (B) mouse brain tissue extracts.
Application
Blots were probed with p-Tau (Ser 396): preincubated with cognate phosphorylated peptide (A) and p-Tau (Ser 396): (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded rat hippocampal tissue showing cytoskeletal staining.| Application Notes | recommended for detection of Ser 396 phosphorylated Tau of human origin, correspondingly phosphorylated Ser 687 of mouse origin and correspondingly phosphorylated Ser 706 of rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
