Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nucleolar and nuclear staining of squamous epithelial cells.Application
Western blot analysis of C23 expression in Jurkat nuclear extract.Application
Western blot analysis of C23 expression in KNRK nuclear extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization.| Product Name | C23 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 271-520 mapping to an internal region of C23 of human origin |
| Modification Notes | C23 (nucleolin, NCL) is a eukaryotic nucleolar phosphoprotein that influences synthesis and maturation of ribosomes. C23 localizes to dense fibrillar regions of the nucleolus. It contains four RNA binding domains that interact with pre-rRNA during synthesis. C23 can influence RNA processing, ribosomal gene transcription and nucleolar targeting of ribosomal components. It is known to associate with a variety of proteins, including the nucleolar protein B23. Phosphorylation by Cdc2 and casein kinase II causes translocation of C23 from the nucleolus to the cytoplasm. Mitotic phosphorylated forms of Bcl-2 are present in nuclear structures in prophase Hela cells together with C23 and Ki-67. Retinoic acid-induced apoptosis leads to C23 down-regulation and Bcl-2 mRNA instability. C23 binds the human telomerase reverse transcriptase subunit (TERT) through interactions with its RNA binding domain 4 and carboxyl-terminal RGG domain, and this interaction is critical for the nucleolar localization of human TERT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of C23 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 271-520 mapping to an internal region of C23 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 271-520 mapping to an internal region of C23 of human origin |
| Entrez Gene | 4691 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing nucleolar and nuclear staining of squamous epithelial cells.
Application
Western blot analysis of C23 expression in Jurkat nuclear extract.
Application
Western blot analysis of C23 expression in KNRK nuclear extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nucleolar localization.| Application Notes | recommended for detection of C23 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
