Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells.Application
Western blot analysis of IκB-ζ expression in HeLa nuclear extract (A) and WI 38 whole cell lysate (B).| Product Name | IκB-ζ Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 491-540 mapping within an internal region of IκB-ζ of human origin |
| Modification Notes | I˚B-Ω (also called MAIL-S or INAP) is a member of the I˚B family. It shares a 30% identity with other family members and consists of six ankyrin repeats at its C-terminal. I˚B-Ω accumulates in the nucleus and, in humans, associates with the p50 and p65 subunits of nuclear NF˚B via its ankyrin repeats. The mouse homologue of I˚B-Ω has only been shown to associate with the p50 subunit. I˚B-Ω inhibits DNA binding and activity of the transcription factor NF˚B. Distinct from other I˚B family members, I˚B-Ω is not degraded upon cell stimulation and activation of NF˚B, rather evidence shows that it is up-regulated under these circumstances. This suggests that I˚B-Ω plays a significant role in regulation of NF˚B and that NF˚B may regulate I˚B-Ω in a negative feedback loop. Regulation of NF˚B by I˚B-Ω may differ depending on the species. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IκB-ζ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 491-540 mapping within an internal region of I&kappa B-&zeta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 491-540 mapping within an internal region of IκB-ζ of human origin |
| Entrez Gene | 64332 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing nuclear and cytoplasmic staining of hematopoietic cells.
Application
Western blot analysis of IκB-ζ expression in HeLa nuclear extract (A) and WI 38 whole cell lysate (B).| Application Notes | recommended for detection of IκB-ζ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
