Application
Western blot analysis of ALDH8A1 expression in mouse liver tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.Application
Western blot analysis of ALDH8A1 expression in non-transfected: (A) and mouse ALDH8A1 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).| Product Name | ALDH8A1 Antibody (D-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ALDH8A1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Aldehyde dehydrogenases (ALDHs) mediate the NADP+-dependent oxidation of aldehydes into acids and play an important role in the detoxification of alcohol-derived acetaldehyde, as well as in lipid peroxidation and in the metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH8A1 (aldehyde dehydrogenase 8 family, member A1), also known as ALDH12, is a 487 amino acid protein that localizes to the cytoplasm and belongs to the aldehyde dehydrogenase family. Expressed in kidney and liver, ALDH8A1 converts 9-cis-retinal to 9-cis-retinoic acid. 9-cis-retinoic acid activates retinoid X receptors, a family of nuclear receptors which are involved in regulating multiple signaling pathways. Three isoforms exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ALDH8A1 of mouse and human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of ALDH8A1 of mouse and human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 64577 |
|---|
Application
Western blot analysis of ALDH8A1 expression in mouse liver tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.
Application
Western blot analysis of ALDH8A1 expression in non-transfected: (A) and mouse ALDH8A1 transfected: (B) 293T whole cell lysates and mouse kidney tissue extract (C).| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
