Application
Western blot analysis of PCTAIRE-3 expression in non-transfected: (A) and human PCTAIRE-3 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PCTAIRE-3 expression in HL-60 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Product Name | PCTAIRE-3 Antibody (P-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PCTAIRE-3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with cyclins to phosphorylate key substrates involved in cell cycle progression. Another family of proteins, Cdk inhibitors, also play a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Members of the Cdk family include Cdk2–Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. PCTAIRE-1, PCTAIRE-2 and PCTAIRE-3 comprise a subfamily of Cdc2-related serine/threonine kinases. PCTAIRE-1, which is expressed primarily in mammalian brain, interacts with a variety of proteins, and is thought to be part of a multiple signal transduction cascade. PCTAIRE-2, also with expression in brain, may be important in terminally differentiated neurons. The human PCTAIRE-3 gene maps to chromosome 1q32.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PCTAIRE-3 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PCTAIRE-3 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 5129 |
|---|
Application
Western blot analysis of PCTAIRE-3 expression in non-transfected: (A) and human PCTAIRE-3 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PCTAIRE-3 expression in HL-60 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear and cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
