Application
Western blot analysis of ALDH5A1 expression in non-transfected: (A) and human ALDH5A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ALDH5A1 expression in non-transfected (A) and human ALDH5A1 transfected (B) 293 whole cell lysates.Application
Western blot analysis of ALDH5A1 expression in non-transfected: (A) and human ALDH5A1 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Product Name | ALDH5A1 Antibody (B-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of ALDH5A1 of human origin by WB, IP, IF and IHC(P) |
| Modification Notes | Aldehyde dehydrogenases (ALDHs) mediate the NADP+-dependent oxidation of aldehydes into acids and play an important role in the detoxification of alcohol-derived acetaldehyde, as well as in lipid peroxidation and in the metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH5A1 (aldehyde dehydrogenase 5 family, member A1), also known as SSDH or SSADH, is a 535 amino acid protein that localizes to the mitochondria and belongs to the aldehyde dehydrogenase family. Expressed in a variety of tissues, including liver, heart, lung, brain, kidney and placenta, ALDH5A1 is required for gamma-aminobutyric acid (GABA) recycling from the synaptic cleft. Mutations of ALDH5A1 leads to succinate semialdehyde dehydrogenase deficiency (SSADH deficiency) that is characterized by severe ataxia and by mildly retarded psychomotor development. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of ALDH5A1 of human origin by WB IP IF and IHC(P) |
|---|---|
| Tissue Specificity | recommended for detection of ALDH5A1 of human origin by WB, IP, IF and IHC(P) |
| Entrez Gene | 7915 |
|---|
Application
Western blot analysis of ALDH5A1 expression in non-transfected: (A) and human ALDH5A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ALDH5A1 expression in non-transfected (A) and human ALDH5A1 transfected (B) 293 whole cell lysates.
Application
Western blot analysis of ALDH5A1 expression in non-transfected: (A) and human ALDH5A1 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
