Application
Western blot analysis of Enigma expression in non-transfected: (A) and human Enigma transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of Enigma expression in non-transfected: (A) and human Enigma transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).| Product Name | Enigma Antibody (H-110) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 184-293 mapping within an internal region of Enigma of human origin |
| Modification Notes | Enigma, also known as PDLIM7 (PDZ and LIM domain 7) or LMP1 (LIM mineralization protein), is a 457 amino acid protein that localizes to both the cytoplasm and the cytoskeleton. Expressed ubiquitously with highest expression in skeletal muscle, spleen, lung and fetal liver, Enigma is thought to function as a scaffold on which protein assembly can occur. Enigma contains three LIM zinc-binding domains and one PDZ domain through which it may also act as an adaptor, linking various proteins to actin filaments found in skeletal muscle and non-muscle tissues. Additionally, Enigma is directly involved in the two mechanisms of bone formation, namely direct bone formation (embryonic flat bones mandible and cranium) and endochondral bone formation (embryonic long bone development), and may play a role in bone fracture repair. Six isoforms of Enigma exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Enigma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, canine and equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 184-293 mapping within an internal region of Enigma of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 184-293 mapping within an internal region of Enigma of human origin |
| Entrez Gene | 9260 |
|---|
Application
Western blot analysis of Enigma expression in non-transfected: (A) and human Enigma transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of Enigma expression in non-transfected: (A) and human Enigma transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).| Application Notes | recommended for detection of Enigma of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine, canine and equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
