Application
Western blot analysis of Synoviolin expression in non-transfected: (A) and mouse Synoviolin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Synoviolin expression in non-transfected: (A) and human Synoviolin transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), human Synoviolin transfected 293T: (B) and Ramos (C) whole cell lysates.Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), human Synoviolin transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of Synoviolin expression in mouse kidney tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), mouse Synoviolin transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), mouse Synoviolin transfected 293T: (B) and Hep G2 (C) whole cell lysates.Application
Western blot analysis of Synoviolin expression in non-transfected: (A) and mouse Synoviolin transfected: (B) 293T whole cell lysates.| Product Name | Synoviolin Antibody (A-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Synoviolin of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Modification Notes | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). Synoviolin, also known as SYVN1 (synovial apoptosis inhibitor 1) or HRD1, is a 617 amino acid multi-pass membrane protein that localizes to the endoplasmic reticulum and contains one RING-type zinc finger. Expressed ubiquitously with highest expression in kidney and liver, Synoviolin exists as a homodimer that exhibits E3 ubiquitin-protein ligase activity and is a component of the ER-associated degradation (ERAD) complex, a multi-protein structure that mediates the degradation of misfolded proteins within the ER. Synoviolin is upregulated in patients with rheumatoid arthritis (RA), suggesting a role for Synoviolin in the pathogenesis of RA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Synoviolin of mouse and human origin by WB IP IF IHC(P) ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Synoviolin of mouse and human origin by WB, IP, IF, IHC(P), ELISA |
| Entrez Gene | 84447 |
|---|
Application
Western blot analysis of Synoviolin expression in non-transfected: (A) and mouse Synoviolin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Synoviolin expression in non-transfected: (A) and human Synoviolin transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), human Synoviolin transfected 293T: (B) and Ramos (C) whole cell lysates.
Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), human Synoviolin transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of Synoviolin expression in mouse kidney tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic and membrane localization.
Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), mouse Synoviolin transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of Synoviolin expression in non-transfected 293T: (A), mouse Synoviolin transfected 293T: (B) and Hep G2 (C) whole cell lysates.
Application
Western blot analysis of Synoviolin expression in non-transfected: (A) and mouse Synoviolin transfected: (B) 293T whole cell lysates.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
