Application
Western blot analysis of B7-1 expression in non-transfected: (A) and human B7-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of B7-1 expression in Ramos whole cell lysate.| Product Name | B7-1 Antibody (H-208) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 35-242 representing the extracellular domain of B7-1 of human origin |
| Modification Notes | T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells, bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31% overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counterreceptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of B7-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-242 representing the extracellular domain of B7-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-242 representing the extracellular domain of B7-1 of human origin |
| Entrez Gene | 941 |
|---|
Application
Western blot analysis of B7-1 expression in non-transfected: (A) and human B7-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of B7-1 expression in Ramos whole cell lysate.| Application Notes | recommended for detection of B7-1 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
