Application
Western blot analysis of C5 expression in rat liver tissue extract.Application
Western blot analysis of purified human C5β compliment fusion protein.Application
Western blot analysis of C5β expression in A549 whole cell lysate.| Product Name | C5β Antibody (H-196) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-216 mapping at the N-terminus of C5β of human origin |
| Modification Notes | C3a, C4a and C5a are potent anaphylatoxins that are released during complement activation, a system of ligand-surface protein interactions specific to cells of hematopoietic lineage that aids in the elimination of pathogens. Complement C5 precursor contains C5a anaphylatoxin. C3a and C5a secretion correlates with pathophysiological phenotypes such as asthma and bacterial meningitis. Binding of these proteins to their respective G protein-coupled receptors (C3aR, C5aR), which are present on the surface of myeloid leukocytes, induces proinflammatory events such as cellular degranulation, smooth muscle contraction, arachidonic acid metabolism, cytokine release, leukocyte activation and cellular chemotaxis. C5aR utilizes the Ras-Raf-ERK1/2 cascade, couples to Gi/G16 proteins, and is prevalent on the surface of hepatocyte, lung, smooth muscle and endothelial cells. Upon activation, C3aR and C5aR are susceptible to rapid GRK-mediated phosphorylation and Clathrin-coated vesicle targeting. The C5 precursor is first processed by the removal of four basic residues, forming two chains, ? and ?, linked by a disulfide bond. C5 convertase activates C5 by cleaving the ? chain, releasing C5? anaphylatoxin and generating C5?. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of C5 precursor and C5β chain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-216 mapping at the N-terminus of C5&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-216 mapping at the N-terminus of C5β of human origin |
| Entrez Gene | 727 |
|---|
Application
Western blot analysis of C5 expression in rat liver tissue extract.
Application
Western blot analysis of purified human C5β compliment fusion protein.
Application
Western blot analysis of C5β expression in A549 whole cell lysate.| Application Notes | recommended for detection of C5 precursor and C5β chain of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
