Application
Western blot analysis of Nop56 expression in human fetal lung tissue extract.| Product Name | Nop56 Antibody (Q-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Nop56 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | Nop1p (nucleolar protein 1) is a phylogenetically conserved protein essential for efficient processing of pre-rRNA through its association with a class of small nucleolar RNAs during ribosomal biogenesis. Small nucleolar RNAs (snoRNAs) are associated in ribonucleoprotein particles localized to the nucleolus (snoRNPs). Nop1p is structurally and functionally homologous to vertebrate fibrillarin and is essential for viability. The Saccharomyces cerevisiae NOP1 gene encodes a protein resembling the dense fibrillar region of mammalian nucleoli. Nop5p functions with Nop1p in the execution of early pre-rRNA processing steps that lead to formation of 18 S rRNA. In Archaea, fibrillarin and Nop5p comprise the core complex of box C/D snoRNAs, which are responsible for site-specific 2'-hydroxyl methylation of ribosomal and transfer RNAs. Nop56p is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2'-O-methylation of pre-rRNA during its maturation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Nop56 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Nop56 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 10528 |
|---|
Application
Western blot analysis of Nop56 expression in human fetal lung tissue extract.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
