Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of CD55 expression in non-transfected: (A) and human CD55 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD55 expression in human PBL whole cell lysate.Application
Western blot analysis of CD55 expression in non-transfected: (A) and human CD55 transfected: (B) 293 whole cell lysates.Application
Western blot analysis CD55 expression in PC-3 (A) and AML-193 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing membrane staining of alveolar cells and macrophages (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of CD55 expression in CTLL-2 whole cell lysate.| Product Name | CD55 Antibody (H-319) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 35-353 representing mature CD55 of human origin |
| Modification Notes | CD55, also called decay accelerating factor (DAF), is a GPI-anchored single chain glycoprotein (1). CD55 may play a role in protecting cells from complement-mediated lysis by preventing the amplification steps of the complement cascade (2,3). CD55 functions to prevent the assembly of C3 convertase or to accelerate the disassembly of preformed convertase, which blocks formation of the membrane attack complex (4,5). CD55 is expressed on cells in contact with serum, including hematopoietic and many non-hematopoietic cells (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD55 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 35-353 representing mature CD55 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 35-353 representing mature CD55 of human origin |
| Entrez Gene | 1604 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of CD55 expression in non-transfected: (A) and human CD55 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD55 expression in human PBL whole cell lysate.
Application
Western blot analysis of CD55 expression in non-transfected: (A) and human CD55 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis CD55 expression in PC-3 (A) and AML-193 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing membrane staining of alveolar cells and macrophages (low and high magni?fication). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of CD55 expression in CTLL-2 whole cell lysate.| Application Notes | recommended for detection of CD55 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
