Application
Western blot analysis of PTRF expression in NIH/3T3 whole cell lysate.| Product Name | PTRF Antibody (O-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of PTRF of mouse origin by WB, IP and ELISA |
| Modification Notes | The termination of transcription by RNA polymerase I (Pol I) requires the involvement of several proteins, including TTF-1 (Thyroid transcription factor-1) which pauses transcription, thus allowing the Pol I complex to dissociate and release the subsequent pre-rRNA. PTRF (Polymerase I and transcript release factor), also known as FKSG13, is a 390 amino acid protein that is required for the dissociation of the transcription complex. Localized to various places within the cell, including the cell membrane, microsome, nucleus and cytoplasm, PTRF binds the 3’ end of pre-rRNA while simultaneously interacting with Pol I and TFF-1, thus allowing the Pol I complex to release from the template. Three isoforms of PTRF are expressed due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of PTRF of mouse origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of PTRF of mouse origin by WB, IP and ELISA |
| Entrez Gene | 19285 |
|---|
Application
Western blot analysis of PTRF expression in NIH/3T3 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
