Application
Western blot analysis of p-PFK-2 car phosphorylation in non-transfected: (A) and mouse p-PFK-2 car transfected: (B) 293T whole cell lysates.Application
Western blot analysis of p-PFK-2 car phosphorylation in non-transfected: (A) and mouse p-PFK-2 car transfected: (B) 293T whole cell lysates.| Product Name | p-PFK-2 car Antibody (Ser 466) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 466 of PFK-2 car of human origin |
| Modification | Phosphoralated |
| Modification Notes | Phosphofructose kinase-2 (PFK-2) belongs to the phosphoglycerate mutase family and is required for the activation of cellular glycolysis. Within the glycolysis pathway, PFK-2 regulates the synthesis and degradation of fructose 2,6-bisphosphate (F2,6BP) by enzymatically catalyzing the phosphorylation of fructose-6-phosphate to form F2,6BP. F2,6BP functions as a potent activator of 6-phosphofructo-1-kinase that can then activate the glycolysis pathway. Various tissue-specific isoforms of PFK-2 are expressed and they are differentially regulated and function as homodimers. A unique isoform, iPFK-2, is induced following proinflammatory stimuli, and it is also constituitively expressed in a variety of carcinoma cell lines, where it leads to an accumulation of intracellular F2,6BP. Activation of PFK-2 cardiac (PFK-2 car) by insulin results from Ser 466 and Ser 483 phosphorylation and requires a PDK-1-activated protein kinase other than PKB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ser 466 phosphorylated cardiac-type PFK-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 466 of PFK-2 car of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 466 of PFK-2 car of human origin |
| Entrez Gene | 5208 |
|---|
Application
Western blot analysis of p-PFK-2 car phosphorylation in non-transfected: (A) and mouse p-PFK-2 car transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of p-PFK-2 car phosphorylation in non-transfected: (A) and mouse p-PFK-2 car transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Ser 466 phosphorylated cardiac-type PFK-2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
