Application
Western blot analysis of CHIC2 expression in non-transfected 293: (A), human CHIC2 transfected 293T: (B) and AML-193 (C) whole cell lysates.Application
Western blot analysis of CHIC2 expression in AML-193 whole cell lysate.Application
Western blot analysis of CHIC2 expression in Hep G2 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.Application
Western blot analysis of CHIC2 expression in 293T whole cell lysate.| Product Name | CHIC2 Antibody (Z-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of CHIC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Modification Notes | CHIC2 (Cysteine-rich hydrophobic domain 2 protein), also known as BTL (BrX-like translocated in leukemia) and BTL/ETV6 fusion gene, is a 165 amino acid membrane protein whose gene is affected in a chromosomal translocation t(4;12)(q11;p13) occurring in acute myeloid leukemias (AML). CHIC2 is associated with the plasma membrane and vesicular structures, suggesting that it plays a role in regulating exocytosis. The cysteine-rich hydrophobic motif of CHIC2 contains cysteines that are palmitoylated, which is required for membrane association. In AML, the CHIC2 gene recombines with the TEL gene, resulting in a fusion protein containing the complete helix-loop-helix (HLH) and ETS DNA binding domains of TEL, but is transcribed via the CHIC2 promoter. Frequently, in systemic mast cell disease with associated eosinophilia, the gene encoding CHIC2 is deleted and a FIP1L1-PDGFR-å rearrangement is observed, a gene fusion which results in a constitutively active PDGFR-å. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of CHIC2 of mouse rat and human origin by WB IP IF IHC(P) and ELISA also reactive with additional species including canine |
|---|---|
| Tissue Specificity | recommended for detection of CHIC2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine |
| Entrez Gene | 26511 |
|---|
Application
Western blot analysis of CHIC2 expression in non-transfected 293: (A), human CHIC2 transfected 293T: (B) and AML-193 (C) whole cell lysates.
Application
Western blot analysis of CHIC2 expression in AML-193 whole cell lysate.
Application
Western blot analysis of CHIC2 expression in Hep G2 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human intestine tissue showing cytoplasmic localization.
Application
Western blot analysis of CHIC2 expression in 293T whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
