Application
Western blot analysis of Nup50 expression in HeLa whole cell lysate.Application
Western blot analysis of Nup50 expression in Hep G2 whole cell lysate.| Product Name | Nup50 Antibody (V-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Nup50 of human origin by WB, IP and ELISA |
| Modification Notes | Nuclear pore complexes (NPCs) are the channels for the bi-directional movement of macromolecules between the nucleus and cytoplasm, and contain more than 100 different subunits. Many of them belong to a family called nucleoporins, which are characterized by the presence of O-linked N-acetylglucosamine moieties and a distinctive pentapeptide repeat (XFXFG). Nup50 (nucleoporin 50), also known as NPAP60 or NPAP60L (nuclear pore-associated protein 60 kDa-like), is a 468 amino acid nuclear protein that functions as a binding site for export receptor-cargo complexes. Localizing to the nucleoplasmic fibrils of the nuclear pore complex, Nup50 associates with various transport receptor proteins including p27. While ubiquitously expressed, Nup50 is found at highest levels in peripheral blood leukocytes, testis and fetal liver, and contains multiple FG repeats in addition to a single RanBD1 domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Nup50 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Nup50 of human origin by WB, IP and ELISA |
| Entrez Gene | 10762 |
|---|
Application
Western blot analysis of Nup50 expression in HeLa whole cell lysate.
Application
Western blot analysis of Nup50 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
