Application
Western blot analysis of Sprm-1 expression in Hep G2 whole cell lysate.| Product Name | Sprm-1 Antibody (L-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Sprm-1 of human origin by WB, IP and ELISA |
| Modification Notes | Tissue-restricted POU domain transcription factors, which bind octamer or octamer-like gene sequences, play roles in cellular differentiation and the development of several organs. Belonging to the POU transcription factor family, Sprm-1 (Sperm 1 POU domain transcription factor), also known as POU5F2 (POU domain, class 5, transcription factor 2), is a 328 amino acid nuclear protein that contains one homeobox DNA-binding domain and one POU-specific domain. Sprm-1 is a transcription factor that preferentially binds to the octamer motif (5'-ATGTTAAT-3'). In the embryo, Sprm-1 expression is restricted to brain, whereas in the adult it is exclusively expressed in brain, skeletal muscle, lung, heart and germ cells. Homozygous null Sprm-1 mice are subfertile, yet exhibit normal testicular morphology and normal numbers of mobile spermatozoa, suggesting that Sprm-1 plays a regulatory role in the haploid spermatid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Sprm-1 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of Sprm-1 of human origin by WB, IP and ELISA |
| Entrez Gene | 134187 |
|---|
Application
Western blot analysis of Sprm-1 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
