Application
Western blot analysis of SECp43 expression in Jurkat whole cell lysate.| Product Name | SECp43 Antibody (B-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of SECp43 of mouse, rat and human origin by WB, IP and ELISA |
| Modification Notes | SECp43, also known as tRNA (Sec) selenocysteine 1-associated protein 1 or TRNAU1AP, is a 287 amino acid member of the RRM TRSPAP protein family. Localized to the nucleus and cytoplasm, yet found more abundantly in the nucleus, SECp43 contains two RRM (RNA recognition motif) domains. The RRM domain contains two highly conserved regions: a six amino acid, hydrophobic motif (RNP-2) and an octapeptide motif (RNP-1). SECp43 is involved in the early stages of selenocysteine biosynthesis and tRNA charging. It is also involved in the later stages of the cotranslational incorporation of selenocysteine into selenoproteins. Possibly involved in the methylation of tRNA, SECp43 associates with mRNP, SELB, SBP-2 and tRNA. Two isoforms of SECp43 exist as a result of alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of SECp43 of mouse rat and human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of SECp43 of mouse, rat and human origin by WB, IP and ELISA |
| Entrez Gene | 54952 |
|---|
Application
Western blot analysis of SECp43 expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
