Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in non-germinal center.| Product Name | p-NFATc2 Antibody (Ser 213/217/221) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to dually phosphorylated Ser 213, 217 and 221 of NFATc2 of human origin |
| Modification | Phosphoralated |
| Modification Notes | The NFAT (nuclear factor of activated T cells) family of transcription factors regulates cytokine expression in T cells. Members of the family include NFATc1 (NFATc), NFATc2 (NFATp), NFATn, NFATc3 (NFAT4, NFATx) and NFATc4 (NFAT3). Recognition of antigen by the T cell receptor (TCR) eventually activates the calcium-dependent protein phosphatase calcineurin. Once activated, calcineurin stimulates the translocation of NFATc1 (cytoplasmic) from the cytoplasm to the nucleus where it associates with NFATn (nuclear). Like NFATc1, NFATc2 resides in the cytoplasm and translocates to the nucleus subsequent to activation of calcineurin. Once in the nucleus, NFATc2 synergizes with AP-1 transcription factors to initiate transcription of cytokine genes. NFATc3 and NFATc4 share 65% sequence identity with other members of the NFAT family. They are similar to NFATc2 in that they also synergize with the AP-1 family of proteins. NFATc2 is phosphorylated by NFATC-kinase and is inducibly expressed in T lymphocytes after TCR complex activation. The phosphorylated form of NFATc2 localizes to the cytoplasm. It is dephosphorylated by calcineurin and localizes to the nucleus after dephosphorylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ser 213, 217 and 221 triply phosphorylated NFATc2 SP-2 domain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to dually phosphorylated Ser 213 217 and 221 of NFATc2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to dually phosphorylated Ser 213, 217 and 221 of NFATc2 of human origin |
| Entrez Gene | 4773 |
|---|
Application
Immuno-peroxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing cytoplasmic staining of cells in non-germinal center.| Application Notes | recommended for detection of Ser 213, 217 and 221 triply phosphorylated NFATc2 SP-2 domain of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
