| Product Name | Rad27 Antibody (y-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 163-382 mapping at the C-terminus of Rad27 of Saccharomyces cerevisiae origin |
| Modification Notes | Cells utilize mechanisms of mismatch repair (MMR) to maintain the integrity of the genome. DNA single-base mismatches include misincorporation of nucleotides by DNA polymerase, deamination of cytosine to uracil thereby forming U/G mispairs, or recombination between homeologous sequences. The Saccharomyces cerevisiae RAD27 gene encodes the yeast homologue of the mammalian FEN-1 nuclease, which processes Okazaki fragments during DNA lagging-strand synthesis. Rad27 (FEN-1) protein is a single-stranded DNA endonuclease and 5'-3' exonuclease that functions in the msh2-mlh1-pms1-dependent MMR system. The endonuclease activity of Rad27 (FEN-1) reduces recombination events between short sequences. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rad27 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 163-382 mapping at the C-terminus of Rad27 of Saccharomyces cerevisiae origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 163-382 mapping at the C-terminus of Rad27 of Saccharomyces cerevisiae origin |
| Application Notes | recommended for detection of Rad27 of Saccaromyces cerevisiae origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
