Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.Application
Western blot analysis of SIK1 expression in non-transfected 293: (A), human SIK1 transfected 293: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of SIK1 expression in non-transfected 293T: (A), human SIK1 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of SIK1 expression in HeLa (A), Jurkat (B) and HEK293 (C) whole cell lysates.| Product Name | SIK1 Antibody (Y-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of SIK1 of human origin |
| Modification Notes | The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. SIK1 (salt-inducible kinase 1), also known as SNF1LK or MSK, is a 783 amino acid protein that contains one UBA domain and one protein kinase domain and belongs to the Ser/Thr protein kinase family. Localized to both the nucleus and the cytoplasm, SIK1 uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation. Additionally, SIK1 has a potential role in regulation of the G2/M cell cycle transition, as well as in inhibitory control of CREB protein function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SIK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with other SNF family members also reactive with additional species, including equine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of SIK1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of SIK1 of human origin |
| Entrez Gene | 150094 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islets of Langerhans and glandular cells.
Application
Western blot analysis of SIK1 expression in non-transfected 293: (A), human SIK1 transfected 293: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of SIK1 expression in non-transfected 293T: (A), human SIK1 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of SIK1 expression in HeLa (A), Jurkat (B) and HEK293 (C) whole cell lysates.| Application Notes | recommended for detection of SIK1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with other SNF family members also reactive with additional species, including equine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
