Application
Western blot analysis of UBE2F expression in HL-60 whole cell lysate.Application
Western blot analysis of UBE2F expression in non-transfected: (A) and human UBE2F transfected: (B) 293 whole cell lysates.Application
Western blot analysis of UBE2F expression in Jurkat whole cell lysate.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | UBE2F Antibody (C-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of UBE2F of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). UBE2F (ubiquitin-conjugating enzyme E2F), also known as NCE2, is a 185 amino acid protein that belongs to the E2 family of ubiquitin conjugating enzymes. UBE2F functions to accept the ubiquitin-like protein NEDD8 from an E1 complex and to catalyze the ATP-dependent attachment of NEDD8 to other proteins, playing a role in the pathway of protein degradation. The gene encoding UBE2F maps to chromosome 2, which encodes over 1,400 genes and comprises nearly 8% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of UBE2F of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of UBE2F of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 140739 |
|---|
Application
Western blot analysis of UBE2F expression in HL-60 whole cell lysate.
Application
Western blot analysis of UBE2F expression in non-transfected: (A) and human UBE2F transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of UBE2F expression in Jurkat whole cell lysate.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
