Application
Western blot analysis of GK2 expression in HCT 116 whole cell lysate.Application
Western blot analysis of GK2 expression in non-transfected 293T: (A) and HeLa (B) whole cell lysates.Application
Western blot analysis of GK2 expression in non-transfected: (A) and human GK2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of GK2 expression in mouse colon tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Product Name | GK2 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GK2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | GK2 (glycerol kinase 2), also known as GKP2 or GKTA, is a 553 amino acid protein that belongs to the FGGY kinase family and is involved in the pathway of glycerol degradation. Localized to the outer membrane of the mitochondrion and expressed at high levels in testis, GK2 functions to catalyze the ATP-dependent conversion of glycerol to glycerol 3-phosphate. Via its catalytic activity, GK2 plays an essential role in the regulation of glycerol uptake and metabolism. The gene encoding GK2 maps to chromosome 4, which encodes nearly 6% of the human genome and has the largest gene deserts (regions of the genome with no protein encoding genes) of all of the human chromosomes. Defects in some of the genes located on chromosome 4 are associated with Huntington's disease, Ellis-van Creveld syndrome, methylmalonic acidemia and polycystic kidney disease. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GK2 of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GK2 of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 2712 |
|---|
Application
Western blot analysis of GK2 expression in HCT 116 whole cell lysate.
Application
Western blot analysis of GK2 expression in non-transfected 293T: (A) and HeLa (B) whole cell lysates.
Application
Western blot analysis of GK2 expression in non-transfected: (A) and human GK2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of GK2 expression in mouse colon tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
