Application
Western blot analysis of CD64 expression in RAW 264.7 whole cell lysate.Application
Western blot analysis of CD64 expression in non-transfected: (A) and human CD64 transfected: (B) 293T whole cell lysates.| Product Name | CD64 Antibody (H-250) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 125-374 of CD64 of human origin |
| Modification Notes | Three different classes of IgG Fc receptors have been described: Fc©RI (CD64), Fc©RII (CD32) and Fc©RIII (CD16). The low affinity receptors, CD64 and CD16, have a putative role in mediating humoral immune responses. CD64 is a surface glycoprotein with high affinity for monomeric IgG, is expressed constitutively on monocytes and macrophages, and can be induced in neutrophils subsequent to IFN-© stimulation. CD64 plays a putative role in the initiation of cell-mediated cytotoxicity. Thus far, three genes encoding four distinct CD64 transcripts have been described. CD64 has been shown to associate with signal transducing subunit of the high affinity IgE receptor. Src family kinases Hck and Lyn show increased kinase activity and will co-immunoprecipitate with CD64 subsequent to receptor cross linking. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD64 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 125-374 of CD64 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 125-374 of CD64 of human origin |
| Entrez Gene | 2209 |
|---|
Application
Western blot analysis of CD64 expression in RAW 264.7 whole cell lysate.
Application
Western blot analysis of CD64 expression in non-transfected: (A) and human CD64 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of CD64 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
